Origins of the reassortant 2009 pandemic influenza virus through proteotyping with mass spectrometry
Identifieur interne : 000765 ( Main/Exploration ); précédent : 000764; suivant : 000766Origins of the reassortant 2009 pandemic influenza virus through proteotyping with mass spectrometry
Auteurs : Neil D. Fernandes [Australie] ; Kevin M. Downard [Australie]Source :
- Journal of Mass Spectrometry [ 1076-5174 ] ; 2014-01.
Abstract
The application of a proteotyping approach employing high resolution mass spectrometry based is shown to be able to determine the gene origin of all major viral proteins in a triple reassortant pandemic 2009 influenza strain. Key to this approach is the identification of unique swine‐host‐specific signature and indicator peptides that are characteristic of influenza viruses circulating in North American and Eurasian swine herds in the years prior to the 2009 influenza pandemic. These swine‐and human pandemic‐specific signatures enable the origins of viral proteins in a clinical virus specimen to be determined and such strains to be rapidly and directly differentiated from other co‐circulating seasonal influenza viruses from the same period. The proteotyping strategy offers advantages over traditional RT‐PCR‐based approaches that are currently the mainstay of influenza surveillance at the molecular level. Copyright © 2014 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/jms.3310
Affiliations:
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Key to this approach is the identification of unique swine‐host‐specific signature and indicator peptides that are characteristic of influenza viruses circulating in North American and Eurasian swine herds in the years prior to the 2009 influenza pandemic. These swine‐and human pandemic‐specific signatures enable the origins of viral proteins in a clinical virus specimen to be determined and such strains to be rapidly and directly differentiated from other co‐circulating seasonal influenza viruses from the same period. The proteotyping strategy offers advantages over traditional RT‐PCR‐based approaches that are currently the mainstay of influenza surveillance at the molecular level. Copyright © 2014 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Australie</li></country><region><li>Nouvelle-Galles du Sud</li></region><settlement><li>Sydney</li></settlement><orgName><li>Université de Sydney</li></orgName></list><tree><country name="Australie"><region name="Nouvelle-Galles du Sud"><name sortKey="Fernandes, Neil D" sort="Fernandes, Neil D" uniqKey="Fernandes N" first="Neil D." last="Fernandes">Neil D. Fernandes</name></region><name sortKey="Downard, Kevin M" sort="Downard, Kevin M" uniqKey="Downard K" first="Kevin M." last="Downard">Kevin M. Downard</name><name sortKey="Downard, Kevin M" sort="Downard, Kevin M" uniqKey="Downard K" first="Kevin M." last="Downard">Kevin M. Downard</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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